LOCUS Exported 15392 bp ds-DNA circular SYN 06-JAN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pC13N-dCas9-BFP-KRAB (151217 seq ver #G8) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15392) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 6, 2019 from SnapGene Viewer 3.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15392 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 9..42 /bound_moiety="Cre recombinase" /note="lox2272" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." promoter 52..1666 /note="CAG Promoter" intron 1673..1766 /note="Mini-Intron" misc_feature 1759..1773 /note="Feature 30" misc_feature 1769..1774 /note="Bsrg1 site used for cloning" misc_feature 1775..1780 /note="part of primer" misc_feature 1781..6955 /note="PCR product" misc_feature 1781..1801 /note="Feature 27" CDS 1787..6955 /codon_start=1 /label=dCas9-HA-NLS-BFP-KRAB /note="dCas9-HA-NLS-BFP-KRAB" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGDAYPYDVPDYASLGSGSPKKKRKVEDPKKKRKVDG IGSGSNGSSGSSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGG PLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDT SLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGG SHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPS KLGHKLNGGGGGMDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENY KNLVSLGYQLTKPDVILRLEKGEEP" misc_feature 5894..5920 /label=HA /note="HA" misc_feature 5939..5959 /label=SV40 NLS /note="SV40 NLS" misc_feature 5945..5959 /label=NLS /note="NLS" misc_feature 6935..6955 /note="Feature 31" misc_feature 6957..6971 /note="Feature 32" misc_feature 6965..7491 /label=Rabbit beta-globin polyA signal /note="Rabbit beta-globin polyA signal" protein_bind complement(7498..7531) /bound_moiety="Cre recombinase" /note="loxP511" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GTATACAT). loxP511 sites are compatible with each other, but incompatible with wild-type loxP sites." misc_feature 7564..8774 /label=chick cHS4 insulator /note="chick cHS4 insulator" misc_feature 8783..9578 /label=C13T1-RA /note="C13T1-RA" CDS 9675..9743 /label=LacZ alpha /note="LacZ alpha" CDS 10095..10904 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 11082..11670 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 11958..11979 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 11994..12024 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 12032..12048 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 12093..12924 /label=C13-LA /note="C13-LA" protein_bind 12933..12966 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 12967..12992 /note="Splice acceptor" CDS 13016..13069 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 13073..13864 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 13899..14123 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" protein_bind 14124..14157 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 14158..15368 /label=chick cHS4 insulator(1) /note="chick cHS4 insulator(1)" ORIGIN 1 cggtcgacat aacttcgtat aggatacttt atacgaagtt atcaattcag gctagttatt 61 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat 121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa 181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg 241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc 301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct 361 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg 421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt 481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc 541 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg 601 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc 661 ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg 721 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt 781 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt 841 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg 901 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc 961 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg 1021 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg 1081 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg 1141 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca 1201 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg 1261 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg 1321 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg 1381 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat 1441 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa 1501 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg 1561 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg 1621 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc 1681 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt 1741 gctgtctcat cattttggca aagaattgtg tacaactagt gccaccatgg acaagaagta 1801 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta 1861 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa 1921 gaacctgatc ggcgccctgc tgttcgacag cggagaaaca gccgaggcca cccggctgaa 1981 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat 2041 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt 2101 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga 2161 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag 2221 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg 2281 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt 2341 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg 2401 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct 2461 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag 2521 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca 2581 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca 2641 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat 2701 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata 2761 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga 2821 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acatcgatgg 2881 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg 2941 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt 3001 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg 3061 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac 3121 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat 3181 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg 3241 cgccagcgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga 3301 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtaca acgagctgac 3361 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa 3421 aaaagccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa 3481 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga 3541 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga 3601 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact 3661 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga 3721 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa 3781 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc 3841 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa 3901 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc 3961 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga 4021 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag 4081 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga 4141 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca 4201 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga 4261 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag 4321 ctttctgaag gacgactcca tcgataacaa agtgctgact cggagcgaca agaaccgggg 4381 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcgcca 4441 gctgctgaat gccaagctga ttacccagag gaagttcgac aatctgacca aggccgagag 4501 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg 4561 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga 4621 gaacgacaaa ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga 4681 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca 4741 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga 4801 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag 4861 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt 4921 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac 4981 aaacggcgaa acaggcgaga tcgtgtggga taagggccgg gactttgcca ccgtgcggaa 5041 agtgctgtct atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt 5101 cagcaaagag tctatcctgc ccaagaggaa cagcgacaag ctgatcgcca gaaagaagga 5161 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt 5221 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg 5281 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa 5341 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga 5401 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga 5461 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct 5521 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaaacacta 5581 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc 5641 taatctggac aaggtgctga gcgcctacaa caagcacaga gacaagccta tcagagagca 5701 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa 5761 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc 5821 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct 5881 gggaggcgac gcctatccct atgacgtgcc cgattatgcc agcctgggca gcggctcccc 5941 caagaaaaaa cgcaaggtgg aagatcctaa gaaaaagcgg aaagtggacg gcattggtag 6001 tgggagcaac ggcagcagcg gatccagcga gctgattaag gagaacatgc acatgaagct 6061 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa 6121 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt 6181 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac 6241 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt 6301 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg 6361 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat 6421 gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg 6481 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa 6541 catcaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta 6601 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca 6661 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa 6721 tggcggtggc ggagggatgg atgctaagtc actaactgcc tggtcccgga cactggtgac 6781 cttcaaggat gtatttgtgg acttcaccag ggaggagtgg aagctgctgg acactgctca 6841 gcagatcgtg tacagaaatg tgatgctgga gaactataag aacctggttt ccttgggtta 6901 tcagcttact aagccagatg tgatcctccg gttggagaag ggagaagagc cctagacgcg 6961 tgaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc 7021 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat 7081 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 7141 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa 7201 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat 7261 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc 7321 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt 7381 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct 7441 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat caccggtata 7501 acttcgtata atgtatacta tacgaagtta tggtacctta attaagttaa catgcatagt 7561 actgagctca cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc 7621 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca 7681 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct 7741 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa 7801 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca 7861 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca 7921 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc 7981 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc 8041 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca 8101 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca 8161 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc 8221 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata 8281 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa 8341 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc 8401 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggactgggac 8461 aggcagctgg agaattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa 8521 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc 8581 cctcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc 8641 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca 8701 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag 8761 tcagtgagaa tattgtttaa actcctcatc cttccaacat aaatatattt tgggattata 8821 tcaacattca atgttactta aagtgacctt gtaaatattt tcacaactga gccatgtttg 8881 atttgtatac ttatgtttac tttactgttt ttcctgaagt taataattgc cttgaattta 8941 tttatttctt taaaaatgtt tcattactca ggactgtagt ttacattacg attctttgtg 9001 ttatacagtt gatgggtttc ttttctttct taatttcttt aaaaaataga gatggggtct 9061 tactatatta cccaggctgg tcttgaagtc ctgggctcaa gtgatcttcc tgtctcagcc 9121 taccaagtag ctgagactat aggtgcaaaa aagccactat acctggctag tttacaggtt 9181 ttaacaaatg cattatgcca cgtatccatt attacaggat cacacaagat attttcatta 9241 ccctgaaagc atccctgtgt tccaccaatt catcctgcct ccatgagccg ctggcaacca 9301 ctgatctcta tagttttgcc ttttctaaaa tgtcatataa ttggaatcat acagtctgta 9361 gcattttcag actagctttt aaaatttggc aatatgcatt taaggttcct ccttaaatgt 9421 gaagggcata gccaatgtgg cttgatagct catttctttt tattggtgaa tatttcattg 9481 tctggatgtt ccacagtttg tttatccatt cacctattca atttgctttt tttctgtgta 9541 tctatcacta attcaatact ggactctcca acagagccgt ttaaacactg gccgtcgttt 9601 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 9661 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 9721 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg 9781 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 9841 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 9901 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 9961 cgtcatcacc gaaacgcgcg atgcagctct ggcccgtgtc tcaaaatctc tgatgttaca 10021 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 10081 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca 10141 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg 10201 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca 10261 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat 10321 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca 10381 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg 10441 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta 10501 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata 10561 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag 10621 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg 10681 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg 10741 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg 10801 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata 10861 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt 10921 ggttgtaaca ttattcagat tgggcttgat ttaaaacttc atttttaatt taaaaggatc 10981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 11041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 11101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 11161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 11221 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 11281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 11341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 11401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 11461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 11521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 11581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 11641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 11701 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 11761 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 11821 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 11881 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 11941 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 12001 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga 12061 aacagctatg accatgatta cgccgcggcc gcagcaggag aatcatgcca atgggccaat 12121 atacattctg acccacagtt tcataataaa ataaaatggt tgtggttgta agccactatg 12181 tttcagagtg gtttgttaca cagcaataaa taactaatat agtaggcata ccatcaagtc 12241 caaagtaggt agagaagaat gtaaatagca gagcaaaaca gcatgactgg tggctgggag 12301 gcttaaaact gggacaggat cagagtcatg aaagaagtca aagaaatggt tcagaagtaa 12361 ggctgagact gacttacaaa agctgaaagt ccctttaagt tggtgtttgg tgcattggca 12421 ggggcaggta tggtgactta aaagagccat gctcaacaag atcaagcaca acacaatcac 12481 gggtcacccc agcagacctt agcgagtcta gccatttctt tggtggtggt cacagtcatg 12541 cttcagccca gtttccactt ggacaaatgg tacatatttt caatgagatg aaaattaaga 12601 tacaatccat gtgctcagag agtgatcaca gctctgacta aacactgtgc cccaaagtgt 12661 tgaggaattg ggaaaaccta gctgagttag tggtctcttt tctgttacaa taaagctcat 12721 aatgaaaatt agccttcttt gttcttcccc aagtctttct ttctagacga aactactttc 12781 aactgtttta acttccttac tgttaacttc catatttcta gatagtatgg tcagactgtt 12841 atttcttgac ttttaaatgt aagatattat ctactgactt ccttctatgt aagatgagga 12901 ttgagctctc ttacccttct cccagcggcc gcataacttc gtatagcata cattatacga 12961 agttatctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg 13021 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggattgaaca 13081 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 13141 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 13201 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 13261 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 13321 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 13381 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 13441 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 13501 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 13561 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 13621 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 13681 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 13741 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 13801 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 13861 ctaacacctg gtgcatgacc cgcaagcccg gtgcctgact gtgccttcta gttgccagcc 13921 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 13981 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 14041 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 14101 tggggatgcg gtgggctcta tggataactt cgtatagcat acattatacg aagttatgag 14161 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta 14221 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg 14281 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc 14341 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct 14401 ttaggctgaa agagagattt agaatgacag aatcatagaa cggcctgggt tgcaaaggag 14461 cacagtgctc atccagatcc aaccccctgc tatgtgcagg gtcatcaacc agcagcccag 14521 gctgcccaga gccacatcca gcctggcctt gaatgcctgc agggatgggg catccacagc 14581 ctccttgggc aacctgttca gtgcgtcacc accctctggg ggaaaaactg cctcctcata 14641 tccaacccaa acctcccctg tctcagtgta aagccattcc cccttgtcct atcaaggggg 14701 agtttgctgt gacattgttg gtctggggtg acacatgttt gccaattcag tgcatcacgg 14761 agaggcagat cttggggata aggaagtgca ggacagcatg gacgtgggac atgcaggtgt 14821 tgagggctct gggacactct ccaagtcaca gcgttcagaa cagccttaag gataagaaga 14881 taggatagaa ggacaaagag caagttaaaa cccagcatgg agaggagcac aaaaaggcca 14941 cagacactgc tggtccctgt gtctgagcct gcatgtttga tggtgtctgg atgcaagcag 15001 aaggggtgga agagcttgcc tggagagata cagctgggtc agtaggactg ggacaggcag 15061 ctggagaatt gccatgtaga tgttcataca atcgtcaaat catgaaggct ggaaaagccc 15121 tccaagatcc ccaagaccaa ccccaaccca cccaccgtgc ccactggcca tgtccctcag 15181 tgccacatcc ccacagttct tcatcacctc cagggacggt gaccccccca cctccgtggg 15241 cagctgtgcc actgcagcac cgctctttgg agaaggtaaa tcttgctaaa tccagcccga 15301 ccctcccctg gcacaacgta aggccattat ctctcatcca actccaggac ggagtcagtg 15361 agaatattgg cgcgccgaat tctcgcgacg at //