LOCUS Exported 12197 bp ds-DNA circular SYN 06-JAN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMTL_3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12197) AUTHORS Conklin Lab TITLE Direct Submission JOURNAL Exported Jan 6, 2019 from SnapGene Viewer 3.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12197 /organism="synthetic DNA construct" /mol_type="other DNA" source 584..1877 /organism="synthetic DNA construct" /mol_type="other DNA" misc_recomb 1..583 /label=AAV 5' Arm /note="AAV 5' Arm" /note="Common feature added by Mo Mandegar" misc_recomb 584..801 /label=AAV 5' Arm /note="AAV 5' Arm" protein_bind 806..839 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 844..870 /note="Splice Acceptor" /note="Feature added by Mo Mandegar Based on the original Map (https://www.addgene.org/static/data/47/49/fa8f6f8a-af62-11 e0-90fe-003048dd6500.jpeg) The location of the Splice Acceptor site is not clearly defined. However based on the map it is located between the HindIII and AvaI site." CDS 895..948 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 958..1557 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 1595..1819 /note="bGHpolyA" /note="bovine growth hormone polyadenylation signal Common feature added by Mo Mandegar" protein_bind 1823..1856 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." enhancer 1859..1877 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" enhancer 1878..2238 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" enhancer 2240..3487 /label=CAG /note="CAG" misc_signal 3374..9374 /note="SeqVer 3/14/13" intron 3495..3588 /label=Mini-Intron /note="Mini-Intron" /note="Common feature added by Mo Mandegar" misc_signal 3594..4442 /note="SeqVer (TRE F)" /note="Common feature added by Mo Mandegar" misc_signal complement(3594..3754) /note="SeqVer (KRAB R)" misc_feature 3594..3603 /label=Kozak+ATG /note="Kozak+ATG" /note="Common feature added by Mo Mandegar Note: Designating ""cgccaccATG"" as the Kozak+ATG in a number of constructs. This is the most conserved consensues sequence. Works well in HEK and iPS cells. From Wikipedia Dec 2013: The Kozak consensus sequence, Kozak consensus or Kozak sequence, is a sequence which occurs on eukaryotic mRNA and has the consensus (gcc)gccRccAUGG. The Kozak consensus sequence plays a major role in the initiation of the translation process.[1] The sequence was named after the person who brought it to prominence, Marilyn Kozak." CDS 3604..3816 /codon_start=1 /label=krab /note="krab" /translation="DAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLEN YKNLVSLGYQLTKPDVILRLEKGEEP" misc_signal 3667..4543 /note="SeqVer (KRAB F)" /note="Common feature added by Mo Mandegar" CDS 3817..3840 /codon_start=1 /label=krab(1) /note="krab" /translation="GGSGGGSM" CDS 3841..7941 /codon_start=1 /product="dCas9 D10A&H840A dCas9 = ""dead"" cas9 / Catalytically inactive Cas9 Sequence annotation provided by Lei Stanley Qi Common feature added by Mo Mandegar" /note="dCas9" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" misc_signal 3900..4662 /note="SeqVer (Cas F)" misc_signal complement(7128..7910) /note="SeqVer(Cas R)" misc_signal complement(7147..8014) /note="SeqVer (Cas R)" /note="Common feature added by Mo Mandegar" misc_signal complement(7413..8076) /note="SeqVer (SV40 F)" /note="Common feature added by Mo Mandegar" misc_signal complement(7875..8076) /note="SeqVer (mCherryR)" CDS join(7942..7971,7990..8010,8017..8076) /codon_start=1 /label=HA 2x NLS /note="HA 2x NLS" /note="Feature edited by Mo Mandegar This was done based on common features already included in SnapGene and the original annotated map by Stanley Qi and Luke Gilbert" /translation="AYPYDVPDYAPKKKRKVPKKKRKVDGIGSGSNGSSGS" misc_signal 8077..9009 /note="SeqVer 3/14/13" misc_feature 8077..8772 /note="BFP" misc_feature 8773..9009 /note="KRAB" polyA_signal 9018..9544 /label=RBGpolyA /note="RBGpolyA" /note="Common feature added by Mo Mandegar" protein_bind 9567..9600 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_recomb 9643..10477 /label=AAV 3' Arm /note="AAV 3' Arm" rep_origin complement(10525..11113) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11284..12144) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 tgctttctct gaccagcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt 61 ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt 121 cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc 181 cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg 241 tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct 301 gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct 361 tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc 421 gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc 481 ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt 541 catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc 601 ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc 661 ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct 721 gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct 781 gtcccctcca ccccacagtg gggcaataac ttcgtatagc atacattata cgaagttata 841 agcttctgac ctcttctctt cctcccacag ggcctcgaga gatctggcag cggagagggc 901 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag gctcgagatg 961 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 1021 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 1081 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 1141 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 1201 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 1261 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 1321 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 1381 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 1441 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 1501 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct 1561 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 1621 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 1681 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 1741 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 1801 gatgcggtgg gctctatggg tcataacttc gtatagcata cattatacga agttattcga 1861 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 1921 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 1981 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 2041 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 2101 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 2161 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 2221 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc 2281 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg 2341 ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc 2401 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct 2461 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcgggg 2521 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc 2581 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc 2641 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa 2701 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg 2761 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct 2821 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg 2881 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt 2941 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc 3001 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg 3061 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg 3121 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg 3181 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 3241 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 3301 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 3361 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 3421 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 3481 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 3541 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaatgt cgacgccacc 3601 atggatgcta agtcactaac tgcctggtcc cggacactgg tgaccttcaa ggatgtattt 3661 gtggacttca ccagggagga gtggaagctg ctggacactg ctcagcagat cgtgtacaga 3721 aatgtgatgc tggagaacta taagaacctg gtttccttgg gttatcagct tactaagcca 3781 gatgtgatcc tccggttgga gaagggagaa gagcccggcg gttccggcgg agggtcgatg 3841 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc 3901 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac 3961 agcatcaaga agaacctgat cggcgccctg ctgttcgaca gcggagaaac agccgaggcc 4021 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat 4081 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg 4141 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac 4201 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa 4261 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg 4321 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg 4381 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc 4441 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg 4501 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg 4561 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat 4621 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag 4681 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg 4741 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg 4801 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag 4861 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc 4921 tacatcgatg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa 4981 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag 5041 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc 5101 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag 5161 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga 5221 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg 5281 gtggacaagg gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgataagaac 5341 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac 5401 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc 5461 ggcgagcaga aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg 5521 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc 5581 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc 5641 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg 5701 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac 5761 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg 5821 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat 5881 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc 5941 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac 6001 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg 6061 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc 6121 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg 6181 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg 6241 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat 6301 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc 6361 gtgcctcaga gctttctgaa ggacgactcc atcgataaca aagtgctgac tcggagcgac 6421 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac 6481 tactggcgcc agctgctgaa tgccaagctg attacccaga ggaagttcga caatctgacc 6541 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg 6601 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact 6661 aagtacgacg agaacgacaa actgatccgg gaagtgaaag tgatcaccct gaagtccaag 6721 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac 6781 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac 6841 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg 6901 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac 6961 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct 7021 ctgatcgaga caaacggcga aacaggcgag atcgtgtggg ataagggccg ggactttgcc 7081 accgtgcgga aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac cgaggtgcag 7141 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgacaa gctgatcgcc 7201 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat 7261 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa 7321 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt 7381 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac 7441 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag 7501 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac 7561 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag 7621 cacaaacact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc 7681 ctggccgacg ctaatctgga caaggtgctg agcgcctaca acaagcacag agacaagcct 7741 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct 7801 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag 7861 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac 7921 ctgtctcagc tgggaggcga cgcctatccc tatgacgtgc ccgattatgc cagcctgggc 7981 agcggctccc ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg gaaagtggac 8041 ggcattggta gtgggagcaa cggcagcagc ggatccagcg agctgattaa ggagaacatg 8101 cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag 8161 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc 8221 cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc 8281 atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca 8341 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc 8401 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac 8461 ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc 8521 gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat 8581 ctgatcgcaa acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg 8641 cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc 8701 tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg 8761 cacaagctta atggcggtgg cggagggatg gatgctaagt cactaactgc ctggtcccgg 8821 acactggtga ccttcaagga tgtatttgtg gacttcacca gggaggagtg gaagctgctg 8881 gacactgctc agcagatcgt gtacagaaat gtgatgctgg agaactataa gaacctggtt 8941 tccttgggtt atcagcttac taagccagat gtgatcctcc ggttggagaa gggagaagag 9001 ccctagtaac gcgtgaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg 9061 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt 9121 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt 9181 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg 9241 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata 9301 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc 9361 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat 9421 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag 9481 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga 9541 gatccctcga cctgcagccc aagcttataa cttcgtatag catacattat acgaagttat 9601 gcggccgcac cggtatgcat ttaattaaga tatcagcttt actagggaca ggattggtga 9661 cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc 9721 cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc 9781 tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg 9841 agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg 9901 ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt 9961 tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa 10021 acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc 10081 aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc 10141 cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga 10201 atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca 10261 tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag 10321 ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag 10381 gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct 10441 cctgcccctt ccctacaggg gttcctggct ctgctctaag ggcgaattcg ccaggaaccg 10501 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 10561 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 10621 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 10681 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 10741 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 10801 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 10861 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 10921 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 10981 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 11041 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 11101 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 11161 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 11221 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 11281 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 11341 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 11401 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 11461 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 11521 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 11581 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 11641 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 11701 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 11761 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 11821 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 11881 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 11941 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 12001 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 12061 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 12121 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 12181 gggttgaatt cgccctt //