LOCUS Exported 12686 bp ds-DNA circular SYN 06-JAN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMTL_5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12686) AUTHORS Conklin Lab TITLE Direct Submission JOURNAL Exported Jan 6, 2019 from SnapGene Viewer 3.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12686 /organism="synthetic DNA construct" /mol_type="other DNA" source 588..1873 /organism="synthetic DNA construct" /mol_type="other DNA" source 3601..4074 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..801 /note="AAV 5' Arm" protein_bind 806..839 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 844..870 /note="Splice Acceptor" /note="Feature added by Mo Mandegar Based on the original Map (https://www.addgene.org/static/data/47/49/fa8f6f8a-af62-11 e0-90fe-003048dd6500.jpeg) The location of the Splice Acceptor site is not clearly defined. However based on the map it is located between the HindIII and AvaI site." CDS 895..948 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 958..1557 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 1595..1819 /note="bGHpolyA" /note="bovine growth hormone polyadenylation signal Common feature added by Mo Mandegar" protein_bind 1823..1856 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 1859..2238 /note="CMV Enhancer" /note="human cytomegalovirus immediate early enhancer" enhancer 2240..3487 /label=CAG /note="CAG" misc_signal 3374..9863 /note="SeqVer 3/14/13" intron 3495..3588 /label=Mini-Intron /note="Mini-Intron" /note="Common feature added by Mo Mandegar" misc_signal 3594..4931 /note="SeqVer (TRE F)" /note="Common feature added by Mo Mandegar" misc_signal complement(3594..4243) /note="SeqVer (KRAB R)" misc_feature 3595..3600 /note="Kozak" CDS 3601..4074 /codon_start=1 /gene="folA" /product="E. coli dihydrofolate reductase" /note="ecDHFR (R12Y, Y100I)" /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGHVYEQSL PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILER" misc_feature 4075..4089 /note="GSGSG Linker" misc_feature 4093..4326 /note="KRAB" misc_signal 4156..5032 /note="SeqVer (KRAB F)" /note="Common feature added by Mo Mandegar" misc_feature 4327..8430 /note="dCas9" /note="Enzymatically deficient Cas9" misc_signal 4389..5151 /note="SeqVer (Cas F)" misc_signal complement(7617..8399) /note="SeqVer(Cas R)" misc_signal complement(7636..8503) /note="SeqVer (Cas R)" /note="Common feature added by Mo Mandegar" misc_signal complement(7902..8565) /note="SeqVer (SV40 F)" /note="Common feature added by Mo Mandegar" misc_signal complement(8364..8565) /note="SeqVer (mCherryR)" CDS join(8431..8460,8479..8499,8506..8565) /codon_start=1 /label=HA 2x NLS /note="HA 2x NLS" /note="Feature edited by Mo Mandegar This was done based on common features already included in SnapGene and the original annotated map by Stanley Qi and Luke Gilbert" /translation="AYPYDVPDYAPKKKRKVPKKKRKVDGIGSGSNGSSGS" misc_feature 8566..9261 /note="BFP" misc_feature 9262..9498 /note="KRAB" polyA_signal 9507..10033 /label=RBGpolyA /note="RBGpolyA" /note="Common feature added by Mo Mandegar" protein_bind 10056..10089 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_recomb 10132..10966 /label=AAV 3' Arm /note="AAV 3' Arm" rep_origin complement(11014..11602) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11773..12633) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 tgctttctct gaccagcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt 61 ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt 121 cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc 181 cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg 241 tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct 301 gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct 361 tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc 421 gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc 481 ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt 541 catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc 601 ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc 661 ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct 721 gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct 781 gtcccctcca ccccacagtg gggcaataac ttcgtatagc atacattata cgaagttata 841 agcttctgac ctcttctctt cctcccacag ggcctcgaga gatctggcag cggagagggc 901 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag gctcgagatg 961 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 1021 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 1081 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 1141 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 1201 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 1261 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 1321 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 1381 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 1441 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 1501 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatct 1561 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct 1621 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt 1681 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg 1741 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg 1801 gatgcggtgg gctctatggg tcataacttc gtatagcata cattatacga agttattcga 1861 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca 1921 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 1981 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 2041 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 2101 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 2161 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 2221 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc 2281 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg 2341 ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc 2401 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct 2461 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcgggg 2521 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc 2581 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc 2641 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa 2701 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg 2761 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct 2821 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg 2881 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt 2941 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc 3001 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg 3061 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg 3121 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg 3181 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 3241 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 3301 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 3361 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 3421 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 3481 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 3541 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaatgt cgacgccacc 3601 atgatcagtc tgattgcggc gttagcggta gatcgggtta tcggcatgga aaacgccatg 3661 ccgtggaacc tgcctgccga tctcgcctgg tttaaacgca acaccttaaa taaacccgtg 3721 attatgggcc gccatacctg ggaatcaatc ggtcgtccgt tgccaggacg caaaaatatt 3781 atcctcagca gtcaaccgag tacggacgat cgcgtaacgt gggtgaagtc ggtggatgaa 3841 gccatcgcgg cgtgtggtga cgtaccagaa atcatggtga ttggcggcgg tcacgtttac 3901 gaacagagct tgccaaaagc gcaaaaactg tatctgacgc atatcgacgc agaagtggaa 3961 ggcgacaccc atttcccgga ttacgagccg gatgactggg aatcggtatt cagcgaattc 4021 cacgatgctg atgcgcagaa ctctcacagc tattgctttg agattctgga gcggggcagc 4081 ggatccggca tggatgctaa gtcactaact gcctggtccc ggacactggt gaccttcaag 4141 gatgtatttg tggacttcac cagggaggag tggaagctgc tggacactgc tcagcagatc 4201 gtgtacagaa atgtgatgct ggagaactat aagaacctgg tttccttggg ttatcagctt 4261 actaagccag atgtgatcct ccggttggag aagggagaag agcccggcgg ttccggcgga 4321 gggtcgatgg acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg 4381 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc 4441 gaccggcaca gcatcaagaa gaacctgatc ggcgccctgc tgttcgacag cggagaaaca 4501 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg 4561 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc 4621 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc 4681 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg 4741 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg 4801 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac 4861 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa 4921 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag 4981 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc 5041 ggcaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg 5101 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg 5161 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac 5221 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc 5281 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc 5341 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc 5401 tacgccggct acatcgatgg cggagccagc caggaagagt tctacaagtt catcaagccc 5461 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg 5521 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag 5581 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa 5641 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga 5701 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc 5761 gaggaagtgg tggacaaggg cgccagcgcc cagagcttca tcgagcggat gaccaacttc 5821 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc 5881 accgtgtaca acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc 5941 ttcctgagcg gcgagcagaa aaaagccatc gtggacctgc tgttcaagac caaccggaaa 6001 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg 6061 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg 6121 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat 6181 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc 6241 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc 6301 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca 6361 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac 6421 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat 6481 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg 6541 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac 6601 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc 6661 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa 6721 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat 6781 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg 6841 gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgataacaa agtgctgact 6901 cggagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag 6961 atgaagaact actggcgcca gctgctgaat gccaagctga ttacccagag gaagttcgac 7021 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag 7081 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg 7141 atgaacacta agtacgacga gaacgacaaa ctgatccggg aagtgaaagt gatcaccctg 7201 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc 7261 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc 7321 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg 7381 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc 7441 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg 7501 aagcggcctc tgatcgagac aaacggcgaa acaggcgaga tcgtgtggga taagggccgg 7561 gactttgcca ccgtgcggaa agtgctgtct atgccccaag tgaatatcgt gaaaaagacc 7621 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgacaag 7681 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc 7741 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag 7801 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc 7861 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg 7921 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc 7981 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg 8041 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt 8101 gtggaacagc acaaacacta cctggacgag atcatcgagc agatcagcga gttctccaag 8161 agagtgatcc tggccgacgc taatctggac aaggtgctga gcgcctacaa caagcacaga 8221 gacaagccta tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg 8281 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc 8341 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca 8401 cggatcgacc tgtctcagct gggaggcgac gcctatccct atgacgtgcc cgattatgcc 8461 agcctgggca gcggctcccc caagaaaaaa cgcaaggtgg aagatcctaa gaaaaagcgg 8521 aaagtggacg gcattggtag tgggagcaac ggcagcagcg gatccagcga gctgattaag 8581 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc 8641 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc 8701 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc 8761 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag 8821 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag 8881 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc 8941 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg 9001 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc 9061 gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac 9121 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac 9181 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc 9241 aaactggggc acaagcttaa tggcggtggc ggagggatgg atgctaagtc actaactgcc 9301 tggtcccgga cactggtgac cttcaaggat gtatttgtgg acttcaccag ggaggagtgg 9361 aagctgctgg acactgctca gcagatcgtg tacagaaatg tgatgctgga gaactataag 9421 aacctggttt ccttgggtta tcagcttact aagccagatg tgatcctccg gttggagaag 9481 ggagaagagc cctagtaacg cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg 9541 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg 9601 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga 9661 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat 9721 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat 9781 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga 9841 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt 9901 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg 9961 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc 10021 tcttatggag atccctcgac ctgcagccca agcttataac ttcgtatagc atacattata 10081 cgaagttatg cggccgcacc ggtatgcatt taattaagat atcagcttta ctagggacag 10141 gattggtgac agaaaagccc catccttagg cctcctcctt cctagtctcc tgatattggg 10201 tctaaccccc acctcctgtt aggcagattc cttatctggt gacacacccc catttcctgg 10261 agccatctct ctccttgcca gaacctctaa ggtttgctta cgatggagcc agagaggatc 10321 ctgggaggga gagcttggca gggggtggga gggaaggggg ggatgcgtga cctgcccggt 10381 tctcagtggc caccctgcgc taccctctcc cagaacctga gctgctctga cgcggctgtc 10441 tggtgcgttt cactgatcct ggtgctgcag cttccttaca cttcccaaga ggagaagcag 10501 tttggaaaaa caaaatcaga ataagttggt cctgagttct aactttggct cttcaccttt 10561 ctagtcccca atttatattg ttcctccgtg cgtcagtttt acctgtgaga taaggccagt 10621 agccagcccc gtcctggcag ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc 10681 tttgtgagaa tggtgcgtcc taggtgttca ccaggtcgtg gccgcctcta ctccctttct 10741 ctttctccat ccttctttcc ttaaagagtc cccagtgcta tctgggacat attcctccgc 10801 ccagagcagg gtcccgcttc cctaaggccc tgctctgggc ttctgggttt gagtccttgg 10861 caagcccagg agaggcgctc aggcttccct gtcccccttc ctcgtccacc atctcatgcc 10921 cctggctctc ctgccccttc cctacagggg ttcctggctc tgctctaagg gcgaattcgc 10981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 11041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 11101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 11161 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 11221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 11281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 11341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 11401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 11461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 11521 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 11581 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 11641 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 11701 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 11761 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 11821 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 11881 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 11941 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 12001 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 12061 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 12121 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 12181 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 12241 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 12301 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 12361 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 12421 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 12481 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 12541 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 12601 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 12661 catttatcag ggttgaattc gccctt //