LOCUS Exported 12261 bp ds-DNA circular SYN 20-OCT-2017 DEFINITION Clone#4 sequence correct WTC11 iPSCs red after nucleofection. ACCESSION . VERSION . KEYWORDS pRT029_F8 CLYBL-CAG-DHFR-dCas9-BFP-KRAB-NLS-DHFR (1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12261) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 6, 2019 from SnapGene Viewer 3.0.3 http://www.snapgene.com COMMENT This file is created by Vector NTI http://www.invitrogen.com/ FEATURES Location/Qualifiers source 1..12261 /organism="synthetic DNA construct" /mol_type="other DNA" source 4158..10891 /organism="synthetic DNA construct" /mol_type="other DNA" source 4164..10355 /organism="synthetic DNA construct" /mol_type="other DNA" source 4164..4637 /organism="synthetic DNA construct" /mol_type="other DNA" source 9831..10355 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(395..1255) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" misc_feature 1273..2110 /note="PCR #A1 product" misc_feature 1273..2104 /label=C13-LA /note="C13-LA" misc_feature 2090..2445 /note="PCR product" misc_feature 2113..2161 /note="added back in primer" misc_feature 2113..2161 /note="PCR #A2" misc_feature 2113..2138 /note="Splice acceptor" misc_feature 2162..2164 /note="post-splice stop codon for CLYBL gene product" misc_signal 2165..2248 /note="SeqVer (AAV F)" misc_signal 2165..2185 /note="SeqVer (AAV F)" polyA_signal 2186..2410 /note="bGHpolyA" /note="bovine growth hormone polyadenylation signal Common feature added/modified by Mo Mandegar" enhancer 2416..2795 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" enhancer 2797..4044 /label=CAG /note="CAG" /note="Common feature added by Mo Mandegar" intron 4052..4145 /label=Mini-Intron /note="Mini-Intron" /note="Common feature added by Mo Mandegar" misc_feature 4146..4151 /note="SalI site/Kozak sequence" misc_feature 4152..4157 /note="NheI site added for cloning" misc_signal complement(4164..4652) /note="SeqVer (KRAB R)" misc_signal 4164..4652 /note="SeqVer (TRE F)" /note="Common feature added by Mo Mandegar" misc_signal 4164..4652 /note="SeqVer 3/14/13" CDS 4164..4637 /codon_start=1 /gene="folA" /product="E. coli dihydrofolate reductase" /note="ecDHFR (R12Y, Y100I)" /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGHVYEQSL PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILER" misc_feature 4638..4652 /note="GSGSG Linker" misc_feature 4653..4664 /note="GSGGTSAT" misc_feature 4654..4658 /note="part of primer" misc_feature 4659..9830 /note="PCR product" misc_feature 4659..4679 /note="Feature 27" CDS 4665..9830 /codon_start=1 /label=dCas9-HA-NLS-BFP-KRAB /note="dCas9-HA-NLS-BFP-KRAB" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGDAYPYDVPDYASLGSGSPKKKRKVEDPKKKRKVDG IGSGSNGSSGSSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGG PLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDT SLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGG SHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPS KLGHKLNGGGGGMDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENY KNLVSLGYQLTKPDVILRLEKGEEP" misc_feature 4665..8768 /note="dCas9" misc_feature complement(8188..8937) /note="QB4721" misc_feature 8772..8798 /label=HA /note="HA" misc_feature 8817..8837 /label=SV40 NLS /note="SV40 NLS" misc_feature 8844..8864 /note="SV40 NLS" misc_feature 8904..9599 /note="BFP" misc_feature 9600..9830 /note="KRAB" CDS 9831..9878 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /note="nucleoplasmin NLS" /translation="KRPAATKKAGQAKKKK" CDS 9879..10355 /codon_start=1 /gene="folA" /product="E. coli dihydrofolate reductase" /note="ecDHFR" /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR" misc_feature 10365..10891 /label=Rabbit beta-globin polyA signal /note="Rabbit beta-globin polyA signal" misc_signal complement(10892..10894) /note="SeqVer (SV40 F)" misc_signal complement(10892..10894) /note="SeqVer (WT AAV R)" misc_feature 10898..11693 /label=C13T1-RA /note="C13T1-RA" misc_recomb 11694..11849 /label=AAV 3' Arm /note="AAV 3' Arm" /note="Common feature added by Mo Mandegar" rep_origin complement(11897..224) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 61 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 301 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 361 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 421 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 481 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 541 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 601 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 661 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 721 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 781 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 841 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 901 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 961 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 1021 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 1081 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 1141 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 1201 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 1261 tcctttttca atagcaggag aatcatgcca atgggccaat atacattctg acccacagtt 1321 tcataataaa ataaaatggt tgtggttgta agccactatg tttcagagtg gtttgttaca 1381 cagcaataaa taactaatat agtaggcata ccatcaagtc caaagtaggt agagaagaat 1441 gtaaatagca gagcaaaaca gcatgactgg tggctgggag gcttaaaact gggacaggat 1501 cagagtcatg aaagaagtca aagaaatggt tcagaagtaa ggctgagact gacttacaaa 1561 agctgaaagt ccctttaagt tggtgtttgg tgcattggca ggggcaggta tggtgactta 1621 aaagagccat gctcaacaag atcaagcaca acacaatcac gggtcacccc agcagacctt 1681 agcgagtcta gccatttctt tggtggtggt cacagtcatg cttcagccca gtttccactt 1741 ggacaaatgg tacatatttt caatgagatg aaaattaaga tacaatccat gtgctcagag 1801 agtgatcaca gctctgacta aacactgtgc cccaaagtgt tgaggaattg ggaaaaccta 1861 gctgagttag tggtctcttt tctgttacaa taaagctcat aatgaaaatt agccttcttt 1921 gttcttcccc aagtctttct ttctagacga aactactttc aactgtttta acttccttac 1981 tgttaacttc catatttcta gatagtatgg tcagactgtt atttcttgac ttttaaatgt 2041 aagatattat ctactgactt ccttctatgt aagatgagga ttgagctctc ttacccttct 2101 cccagcggcc gcctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg 2161 atagaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 2221 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 2281 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 2341 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 2401 ggctctatgg gtctcgacat tgattattga ctagttatta atagtaatca attacggggt 2461 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 2521 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 2581 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 2641 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 2701 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 2761 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct 2821 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat 2881 tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc 2941 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg 3001 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 3061 gcgcgcggcg ggcggggagt cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc 3121 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg 3181 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt 3241 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg 3301 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc 3361 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga 3421 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg 3481 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct 3541 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg 3601 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg 3661 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg 3721 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta 3781 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg 3841 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa 3901 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca 3961 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt 4021 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt 4081 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa 4141 agaatgtcga cgctagcgcc accatgatca gtctgattgc ggcgttagcg gtagatcggg 4201 ttatcggcat ggaaaacgcc atgccgtgga acctgcctgc cgatctcgcc tggtttaaac 4261 gcaacacctt aaataaaccc gtgattatgg gccgccatac ctgggaatca atcggtcgtc 4321 cgttgccagg acgcaaaaat attatcctca gcagtcaacc gagtacggac gatcgcgtaa 4381 cgtgggtgaa gtcggtggat gaagccatcg cggcgtgtgg tgacgtacca gaaatcatgg 4441 tgattggcgg cggtcacgtt tacgaacaga gcttgccaaa agcgcaaaaa ctgtatctga 4501 cgcatatcga cgcagaagtg gaaggcgaca cccatttccc ggattacgag ccggatgact 4561 gggaatcggt attcagcgaa ttccacgatg ctgatgcgca gaactctcac agctattgct 4621 ttgagattct ggagcggggc agcggatccg gcactagtgc caccatggac aagaagtaca 4681 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca 4741 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga 4801 acctgatcgg cgccctgctg ttcgacagcg gagaaacagc cgaggccacc cggctgaaga 4861 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct 4921 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc 4981 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg 5041 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca 5101 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg 5161 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca 5221 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg 5281 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga 5341 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc 5401 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc 5461 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt 5521 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc 5581 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg 5641 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga 5701 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac atcgatggcg 5761 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca 5821 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg 5881 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc 5941 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct 6001 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga 6061 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg 6121 ccagcgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga 6181 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtacaac gagctgacca 6241 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa 6301 aagccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag 6361 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc 6421 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact 6481 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt 6541 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca 6601 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc 6661 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg 6721 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag 6781 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca 6841 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg 6901 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag 6961 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag 7021 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc 7081 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc 7141 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct 7201 ttctgaagga cgactccatc gataacaaag tgctgactcg gagcgacaag aaccggggca 7261 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcgccagc 7321 tgctgaatgc caagctgatt acccagagga agttcgacaa tctgaccaag gccgagagag 7381 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc 7441 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga 7501 acgacaaact gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt 7561 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg 7621 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa 7681 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg 7741 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt 7801 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa 7861 acggcgaaac aggcgagatc gtgtgggata agggccggga ctttgccacc gtgcggaaag 7921 tgctgtctat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca 7981 gcaaagagtc tatcctgccc aagaggaaca gcgacaagct gatcgccaga aagaaggact 8041 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg 8101 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga 8161 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg 8221 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc 8281 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac 8341 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga 8401 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aaacactacc 8461 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta 8521 atctggacaa ggtgctgagc gcctacaaca agcacagaga caagcctatc agagagcagg 8581 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt 8641 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca 8701 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg 8761 gaggcgacgc ctatccctat gacgtgcccg attatgccag cctgggcagc ggctccccca 8821 agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacggc attggtagtg 8881 ggagcaacgg cagcagcgga tccagcgagc tgattaagga gaacatgcac atgaagctgt 8941 acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc 9001 cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg 9061 ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc 9121 agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca 9181 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct 9241 gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc 9301 agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc 9361 tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca 9421 tcaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact 9481 atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc 9541 acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatg 9601 gcggtggcgg agggatggat gctaagtcac taactgcctg gtcccggaca ctggtgacct 9661 tcaaggatgt atttgtggac ttcaccaggg aggagtggaa gctgctggac actgctcagc 9721 agatcgtgta cagaaatgtg atgctggaga actataagaa cctggtttcc ttgggttatc 9781 agcttactaa gccagatgtg atcctccggt tggagaaggg agaagagccc aaaaggccgg 9841 cggccacgaa aaaggccggc caggcaaaaa agaaaaagat cagtctgatt gcggcgttag 9901 cggtagatca cgttatcggc atggaaaccg tcatgccgtg gaacctgcct gccgatctcg 9961 cctggtttaa acgcaacacc ttaaataaac ccgtgattat gggccgccat acctgggaat 10021 caatcggtcg tccgttgcca ggacgcaaaa atattatcct cagcagtcaa ccgagtacgg 10081 acgatcgcgt aacgtgggtg aagtcggtgg atgaagccat cgcggcgtgt ggtgacgtac 10141 cagaaatcat ggttattggc ggcggtcgcg tttatgaaca gttcttgcca aaagcgcaaa 10201 aactgtatct gacgcatatc gacgcagaag tggaaggcga cacccatttc ccggattacg 10261 agccggatga ctgggaatcg gtattcagcg aattccacga tgctgatgcg cagaactctc 10321 acagctattg ctttgagatt ctggagcggc gataaacgcg tgaattcact cctcaggtgc 10381 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact 10441 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga 10501 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc 10561 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg 10621 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa 10681 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct 10741 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc 10801 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag 10861 tcatagctgt ccctcttctc ttatggagat cgatatctcc tcatccttcc aacataaata 10921 tattttggga ttatatcaac attcaatgtt acttaaagtg accttgtaaa tattttcaca 10981 actgagccat gtttgatttg tatacttatg tttactttac tgtttttcct gaagttaata 11041 attgccttga atttatttat ttctttaaaa atgtttcatt actcaggact gtagtttaca 11101 ttacgattct ttgtgttata cagttgatgg gtttcttttc tttcttaatt tctttaaaaa 11161 atagagatgg ggtcttacta tattacccag gctggtcttg aagtcctggg ctcaagtgat 11221 cttcctgtct cagcctacca agtagctgag actataggtg caaaaaagcc actatacctg 11281 gctagtttac aggttttaac aaatgcatta tgccacgtat ccattattac aggatcacac 11341 aagatatttt cattaccctg aaagcatccc tgtgttccac caattcatcc tgcctccatg 11401 agccgctggc aaccactgat ctctatagtt ttgccttttc taaaatgtca tataattgga 11461 atcatacagt ctgtagcatt ttcagactag cttttaaaat ttggcaatat gcatttaagg 11521 ttcctcctta aatgtgaagg gcatagccaa tgtggcttga tagctcattt ctttttattg 11581 gtgaatattt cattgtctgg atgttccaca gtttgtttat ccattcacct attcaatttg 11641 ctttttttct gtgtatctat cactaattca atactggact ctccaacaga gccgtcccgc 11701 ttccctaagg ccctgctctg ggcttctggg tttgagtcct tggcaagccc aggagaggcg 11761 ctcaggcttc cctgtccccc ttcctcgtcc accatctcat gcccctggct ctcctgcccc 11821 ttccctacag gggttcctgg ctctgctcta agggcgaatt cgccaggaac cgtaaaaagg 11881 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 11941 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 12001 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 12061 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 12121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 12181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 12241 tggcagcagc cactggtaac a //