Next-generation shRNA libraries: Datasets

Please cite: 

Kampmann M*, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS* (2015). Next-generation libraries for robust RNA interference-based genome-wide screens. Proceedings of the National Academy of Sciences 112(26):E3384-91. *Corresponding authors.

 

Dataset S1

Processing homogeneity of endogenous human miRNAs. Tab 1: Processing metrics for miRNAs. Tab 2: List of analyzed reference samples.

 

Dataset S2

Oligonucleotide sequences of library testing different shRNA expression formats.

 

Dataset S3

Annotated sequences of vectors for next-generation RNAi libraries.

 

Dataset S4

Genes targeted by human sublibraries.

 

Dataset S5

Genes targeted by mouse sublibraries.

 

Dataset S6

Target sites and Sequence scores of all shRNAs in the human next-generation RNAi library.

 

Dataset S7

Target sites and Sequence scores of all shRNAs in the human next-generation RNAi library.

 

Dataset S8

Gene phenotypes and P values from CTx-DTA pilot screen.