Resources

Bioinformatics

 

 

Datasets

 

3D printables

(replace extension with .stl after download)

Gel combs for size fractionation of genomic DNA from pooled screens

  • Gel comb A (1 ladder well, 2 wide wells with narrow separator):
  • Gel comb B (1 ladder well, 2 wide wells with wide separator): 
  • Gel comb C (1 ladder well, 3 wide wells with wide separators): 

 

Plasmid maps

 

CRISPRi/a plasmids (lentiviral)

  • : sgRNA expression vector compatible with CROP-Seq and imaging screens

CRISPRi/a plasmids (safe-harbor locus targeted)

  • : constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
  • : DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
  • : constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)
  • : double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Fluorescent reporters for mammalian cells (lentiviral)

  • pMK1163: Integrated stress response (ATF4-5'UTR-Venus-IRES-BFP)
  • H53 mNeon-Green-NLS (Ward lab)
  • I2 cytosolic mScarlet (Ward lab)

shRNA expression plasmids (lentiviral)

  • pMK1320: library expression, EF1A promoter, tagBFP
  • pMK1209: library expression, SFFV promoter, tagBFP
  • pMH19: library expression, EF1A promoter, mCherry (thanks to Max Horlbeck)
  • pMH20: library expression, SFFV promoter, mCherry (thanks to Max Horlbeck)
  • pMK1200: Double-shRNA vector, EF1A promoter, mCherry
  • pMK1221: Double-shRNA vector, SFFV promoter, mCherry
  • pMK1224: Double-shRNA vector, SFFV promoter, tagBFP
  • pMK1201: Inducible shRNA expression vector (based on pInducer10, thanks to Steve Elledge)