Resources

Cell Lines

  • Please request the following lines from Coriell:
Parental iPSC line CRISPR machinery Inducible transcription factors for differentiation Coriell link Publication
WTC11 CRISPRi (dCas9-BFP-KRAB) n/a https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AICS-0090-391&PgId=166 We generated this highly characterized line together with the Allen Institute, using the CRISPRi construct described in Tian et al 2019 (PMID 31422865)
WTC11 n/a Ngn2 https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=GM29370&Product=CC  
WTC11 CRISPRi (dCas9-BFP-KRAB) Ngn2 https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=GM29371&Product=CC Tian et al 2019 (PMID 31422865)
NCRM5 CRISPRi (dCas9-BFP-KRAB) Ngn2 https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=GM29369&Product=CC Tian et al 2019 (PMID 31422865)
  • To request other cell lines, please use the following request form.

Plasmids

Please request our plasmids through the Kampmann Lab page on Addgene.
Plasmids for 6 transcription factors for microglial differentiation are on the Gan Lab page on Addgene.

CRISPRbrain Data Commons

CRISPRbrain Kampmann Lab

 

Datasets

Bioinformatics

 

Protocols

Please find the following commonly requested protocols from our lab on protocols.io:

 

3D printables

(replace extension with .stl after download)

Gel combs for size fractionation of genomic DNA from pooled screens:

  • Gel comb A (1 ladder well, 2 wide wells with narrow separator):
  • Gel comb B (1 ladder well, 2 wide wells with wide separator): 
  • Gel comb C (1 ladder well, 3 wide wells with wide separators):