In an article published today in Neuron (open-access preprint on bioRxiv), we present a new technology for large-scale CRISPR-based investigation of gene function in human neurons generated from induced pluripotent stem cells. We uncovered pathways that control neuronal survival and shape. Intriguingly, we found that some housekeeping genes expressed by all cell types have specialized functions in neurons. This technology is the basis for ongoing projects in the Kampmann lab, which aim to uncover mechanisms and new therapies for brain diseases, including Alzheimer's Disease and related dementias and Autism Spectrum Disorder.
Read the UCSF news story describing the results for a broad audience here.
Below is the video UCSF created for it: